The Cellular Imaging Core is comprised of three components, Histology, Microscopy and Image Analysis. The Core continuously explores new technologies and has invested heavily in new equipment and image analysis software. We are committed to maintaining this core as a central resource for neuroscience research at CHB and Harvard Medical School.
Increasingly sophisticated methods to visualize tissue/cellular structure/neuronal circuits and to monitor real-time molecular interactions and biochemical reactions, have contributed to recent progress both in developmental neurobiology and in studying neurodevelopmental disorders. Current advancement has been augmented by access to specific antibodies for immunostaining, cloning of nucleic acids and in situ hybridization, new dyes and cell labeling techniques, in combination with a diverse array of powerful new microscopes. Imaging technologies such as laser scanning confocal microscopy, multiphoton excitation fluorescence, fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), Spinning Disk Confocal microscopy and Array Tomography offer greatly increased resolution of cellular processes and require sophisticated analysis programs.