Survey

Histology Survey

Monday, September 27th, 2010

In preparing the IDDRC grant renewal, we would like to take the opportunity to plan the future of the Histology core and identify new pieces of equipment we need to acquire/replace and define new techniques/services you would like us to provide. Thus we would appreciate your feedback regarding the services and equipment that the Histology Core provides.  Please fill out the survey below and press submit to send your anonymous response.

The purpose of this core is to help IDDRC investigators perform histology-based studies in an effective and cost-effective manner. Your input is essential to keep the core a facility that enhances the research of IDDR investigators.

Attached please find also a description of the Core equipment, services and fees.

Thanks for your input.
Sincerely,

Gabriel Corfas, Ph.D.

Please, respond to these questions:

  • Has your lab used the Core in the last 5 years?
  • Do you see your lab using the core in the next 3 years?

If you have answered yes to any of the above questions, please respond to the next ones.

EQUIPMENT:

1. Has your lab used Core equipment in the past (see list below)?

2. Which equipment have you used, and how often have you use it?

3. Which Core equipment do you foresee your lab using in the next 3 years and how frequently do you envision using each piece of equipment?

4. Which new types of equipment would you like the Core to provide?

Current equipment in the Core includes:

  • Downdraft table for small animal perfusion
  • Automatic staining machine (Leica Autostainer XL)
  • General histology equipment: 3 water baths for paraffin sections, 2 slide warmers, 2 paraffin ovens, 2 vacuum ovens, 2 fume hoods
  • Two rotary microtomes (Reichert-Jung 2030 and 2050)
  • Cryostat (Leica 3050S)
  • Sliding microtome for paraffin, frozen or celloidin sections (Leica SM2000R)
  • Vibrating blade microtome for unfixed and fixed tissue (Leica VT1000S)
  • Microtome knife sharpener (Reichert-Jung 903)
  • Two glass knife makers (LKB 7800 and 7801B)
  • Hybridization oven for in situ hybridizations (Robbins Scientific Model 1000)
  • Dissecting microscope with fiber optics illuminator (Nikon SZ654)
  • Microscope with camera lucida and photo-camera (Zeiss Axioskop)


    • SERVICES

    1. Has your lab used Core services in the past (see list below)?

    2. Which services have you used?

    3. Have you had problems accessing or using the current services?

    4. Which new types of services would you like the Core to provide?

    Current equipment in the Core includes:

    • Rodent perfusion, fixation, Tissues embedding in paraffin, plastic (for EM, array tomography and thick sections), frozen, celloidin.
    • Sectioning of tissues embedded in all the above mentioned materials for light and electron microscopy
    • Tissue staining for histology or EM
    • Consultation for immunostaining, in situ hybrydization and histopathology
    • Knife (cryostat and microtome) sharpening